Prof. dr. L.W. (Leendert) Hamoen
- Science Park 904
- Room number: C3.111
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Postbus 94232
1090 GE Amsterdam
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Research Prof Dr Leendert Hamoen
Cell division, Protein localization, Gene regulation & Novel antimicrobials
Cell division is a vital process, and yet, we still do not know exactly how bacterial cells divide. One of the main projects in the lab is to understand cell division at the molecular level. In this research, we use the well-known bacterial model system Bacillus subtilis, and we focus especially at the interaction between the cell membrane and cell division proteins. One of our breakthroughs was the discovery of the conserved cell division protein SepF. Ultimately, we want to use this knowledge to make a minimal synthetic (bacterial) cell that can grow and divide.
The bacterial cell is surprisingly complex in its organization, and many proteins are exclusively found at the poles. We have discovered that the curvature of the cell membrane can guide proteins to cell poles. However, the mechanism behind this process is in most cases still a mystery.
Bacteria are confronted with an ever changing environment. Cellular adaptation processes to cope with these environmental stresses are carefully regulated. We and others have found that, often, the activation of these cellular differentiation processes (e.g. genetic competence, sporulation and motility) varies greatly between cells in the same population. This enables the population to follow a ‘bet-hedging’ strategy. We study the complex gene regulation processes behind these heterogenic or bimodal cellular differentiation processes.
In our studies we use fluorescence microscopy that enables us to observe single bacterial cells. This technique is also very useful to study the mode of action of novel antimicrobial compounds. Using this technique, we have shown that certain membrane antibiotics will delocalize important membrane proteins in bacteria. Currently, we use our fluorescence microscopy tools to identify and characterize new antimicrobial compounds.
B. subtilis is used in the industry to produce enzymes and vitamins. The bacterium is also used as a probiotic in animal feed, and in fact it is eaten by humans in the form of Natto, which is soybeans fermented by B. subtilis. In collaboration with industrial partners we help to further optimize B. subtilis for applied purposes.
SepF protein rings (Gundogdu et al., 2011, EMBO J) Polar localization of chemoreceptors (Strahl et al., 2015, Nat Commun) Bimodal induction of genetic competence (Gamba et al., 2015, PLoS Genet) Protein delocalization by membrane targeting antibiotics (Strahl & Hamoen, 2010, PNAS) Cell division, Protein localization, Gene regulation & Novel antimicrobials
Cell division is a vital process, and yet, we still do not know exactly how bacterial cells divide. One of the main projects in the lab is to understand cell division at the molecular level. In this research, we use the well-known bacterial model system Bacillus subtilis, and we focus especially at the interaction between the cell membrane and cell division proteins. One of our breakthroughs was the discovery of the conserved cell division protein SepF. Ultimately, we want to use this knowledge to make a minimal synthetic (bacterial) cell that can grow and divide.
The bacterial cell is surprisingly complex in its organization, and many proteins are exclusively found at the poles. We have discovered that the curvature of the cell membrane can guide proteins to cell poles. However, the mechanism behind this process is in most cases still a mystery.
Bacteria are confronted with an ever changing environment. Cellular adaptation processes to cope with these environmental stresses are carefully regulated. We and others have found that, often, the activation of these cellular differentiation processes (e.g. genetic competence, sporulation and motility) varies greatly between cells in the same population. This enables the population to follow a ‘bet-hedging’ strategy. We study the complex gene regulation processes behind these heterogenic or bimodal cellular differentiation processes.
In our studies we use fluorescence microscopy that enables us to observe single bacterial cells. This technique is also very useful to study the mode of action of novel antimicrobial compounds. Using this technique, we have shown that certain membrane antibiotics will delocalize important membrane proteins in bacteria. Currently, we use our fluorescence microscopy tools to identify and characterize new antimicrobial compounds.
B. subtilis is used in the industry to produce enzymes and vitamins. The bacterium is also used as a probiotic in animal feed, and in fact it is eaten by humans in the form of Natto, which is soybeans fermented by B. subtilis. In collaboration with industrial partners we help to further optimize B. subtilis for applied purposes.
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Publications before 2012
Sass, P. M. Josten, K. Famulla, G. Schiffer, H.G. Sahl, L. Hamoen, H. Brötz-Oesterhelt, Antibiotic acyldepsipeptides activate ClpP peptidase to degrade the cell division protein FtsZ. Proc Natl Acad Sci USA, 2011. 108-42: p. 17474-17479.
Hamoen. L.W., Cell division blockage: but this time by a surprisingly conserved protein. Mol Microbiol, 2011. 81-1: p. 1-3.
Gündoğdu, M., Y. Kawai, N. Pavlendova, N. Ogasawara, J. Errington, D. Scheffers, and L.W. Hamoen, Large ring polymers align FtsZ polymers for normal septum formation. EMBO J, 2011. 30-3: p. 617-626.
Strahl, H. and L.W. Hamoen, Membrane potential is important for bacterial cell division. Proc Natl Acad Sci USA, 2010. 107-27: p. 12281-12286.
Oliva, M.A., S. Halbedel, S.M. Freund, P. Dutow, T.A. Leonard, D.B. Veprintsev, L.W. Hamoen*, and J. Lowe*, Features critical for membrane binding revealed by DivIVA crystal structure. EMBO J, 2010. 29-12: p. 1988-2001. *corresponding authors.
Lenarcic, R., S. Halbedel, L. Visser, M. Shaw, L.J. Wu, J. Errington, D. Marenduzzo, and L.W. Hamoen, Localisation of DivIVA by targeting to negatively curved membranes. EMBO J, 2009. 28-15: p.2272-2282.
Lammers, C.R., L.A. Florez, A.G. Schmeisky, S.F. Roppel, U. Mader, L. Hamoen, and J. Stulke, Connecting parts with processes: SubtiWiki and SubtiPathways integrate gene and pathway annotation for Bacillus subtilis. Microbiology, 2010. 156-3: p. 849-859.
Gamba, P., J.W. Veening, N.J. Saunders, L.W. Hamoen, and R.A. Daniel, Two-step assembly dynamics of the Bacillus subtilis divisome. J Bacteriol, 2009. 191-13: p. 4186-4194.
Veening, J.W., E.J. Stewart, T.W. Berngruber, F. Taddei, O.P. Kuipers, and L.W. Hamoen, Bet-hedging and epigenetic inheritance in bacterial cell development. Proc Natl Acad Sci USA, 2008. 105-11: p. 4393-4398.
Veening, J.W., O.A. Igoshin, R.T. Eijlander, R. Nijland, L.W. Hamoen, and O.P. Kuipers, Transient heterogeneity in extracellular protease production by Bacillus subtilis. Mol Syst Biol, 2008. 4-184: p. 184.
Kirstein, J., H. Strahl, N. Moliere, L.W. Hamoen*, and K. Turgay*, Localization of general and regulatory proteolysis in Bacillus subtilis cells. Mol Microbiol, 2008. 70-3: p. 682-694. *corresponding authors.
Claessen, D., R. Emmins, L.W. Hamoen, R.A. Daniel, J. Errington, and D.H. Edwards, Control of the cell elongation-division cycle by shuttling of PBP1 protein in Bacillus subtilis. Mol Microbiol, 2008. 68-4: p. 1029-1046.
Susanna, K.A., A.M. Mironczuk, W.K. Smits, L.W. Hamoen, and O.P. Kuipers, A single, specific thymine mutation in the ComK-binding site severely decreases binding and transcription activation by the competence transcription factor ComK of Bacillus subtilis. J Bacteriol, 2007. 189-13: p. 4718-4728.
Smits, W.K., T.T. Hoa, L.W. Hamoen, O.P. Kuipers, and D. Dubnau, Antirepression as a second mechanism of transcriptional activation by a minor groove binding protein. Mol Microbiol, 2007. 64-2: p. 368-381.
Smits, W.K., C. Bongiorni, J.W. Veening, L.W. Hamoen, O.P. Kuipers, and M. Perego, Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis. Mol Microbiol, 2007. 65-1: p. 103-120.
Nijland, R., C. Lindner, M. van Hartskamp, L.W. Hamoen, and O.P. Kuipers, Heterologous production and secretion of Clostridium perfringens beta-toxoid in closely related Gram-positive hosts. J Biotechnol, 2007. 127-3: p. 361-372.
Nijland, R., R. Heerlien, L.W. Hamoen, and O.P. Kuipers, Changing a single amino acid in Clostridium perfringens beta-toxin affects the efficiency of heterologous secretion by Bacillus subtilis. Appl Environ Microbiol, 2007. 73-5: p. 1586-1593.
Duitman, E.H., D. Wyczawski, L.G. Boven, G. Venema, O.P. Kuipers, and L.W. Hamoen, Novel methods for genetic transformation of natural Bacillus subtilis isolates used to study the regulation of the mycosubtilin and surfactin synthetases. Appl Environ Microbiol, 2007. 73-11: p. 3490-3496.
Veening, J.W., W.K. Smits, L.W. Hamoen, and O.P. Kuipers, Single cell analysis of gene expression patterns of competence development and initiation of sporulation in Bacillus subtilis grown on chemically defined media. J Appl Microbiol, 2006. 101-3: p. 531-541.
Susanna, K.A., F. Fusetti, A.M. Thunnissen, L.W. Hamoen, and O.P. Kuipers, Functional analysis of the competence transcription factor ComK of Bacillus subtilis by characterization of truncation variants. Microbiology, 2006. 152-2: p. 473-483.
Susanna, K.A., C.D. den Hengst, L.W. Hamoen, and O.P. Kuipers, Expression of transcription activator ComK of Bacillus subtilis in the heterologous host Lactococcus lactis leads to a genome-wide repression pattern: a case study of horizontal gene transfer. Appl Environ Microbiol, 2006. 72-1: p. 404-411.
Hamoen, L.W., J.C. Meile, W. de Jong, P. Noirot, and J. Errington, SepF, a novel FtsZ-interacting protein required for a late step in cell division. Mol Microbiol, 2006. 59-3: p. 989-999.
Veening, J.W., L.W. Hamoen, and O.P. Kuipers, Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis. Mol Microbiol, 2005. 56-6: p. 1481-1494.
Smits, W.K., C.C. Eschevins, K.A. Susanna, S. Bron, O.P. Kuipers, and L.W. Hamoen, Stripping Bacillus: ComK auto-stimulation is responsible for the bistable response in competence development. Mol Microbiol, 2005. 56-3: p. 604-614.
Veening, J.W., W.K. Smits, L.W. Hamoen, J.D. Jongbloed, and O.P. Kuipers, Visualization of differential gene expression by improved cyan fluorescent protein and yellow fluorescent protein production in Bacillus subtilis. Appl Environ Microbiol, 2004. 70-11: p. 6809-6815.
Susanna, K.A., A.F. van der Werff, C.D. den Hengst, B. Calles, M. Salas, G. Venema, L.W. Hamoen*, and O.P. Kuipers*, Mechanism of transcription activation at the comG promoter by the competence transcription factor ComK of Bacillus subtilis. J Bacteriol, 2004. 186-4: p. 1120-1128. *corresponding authors.
Lindner, C., R. Nijland, M. van Hartskamp, S. Bron, L.W. Hamoen, and O.P. Kuipers, Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein. J Bacteriol, 2004. 186-4: p. 1097-1105.
Baerends, R.J., W.K. Smits, A. de Jong, L.W. Hamoen, J. Kok, and O.P. Kuipers, Genome2D: a visualization tool for the rapid analysis of bacterial transcriptome data. Genome Biol, 2004. 5-5: p. R37.
Hamoen, L.W., G. Venema, and O.P. Kuipers, Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology, 2003. 149-1: p. 9-17.
Hamoen, L.W., D. Kausche, M.A. Marahiel, D. van Sinderen, G. Venema, and P. Serror, The Bacillus subtilis transition state regulator AbrB binds to the -35 promoter region of comK. FEMS Microbiol Lett, 2003. 218-2: p. 299-304.
Hamoen, L.W. and J. Errington, Polar targeting of DivIVA in Bacillus subtilis is not directly dependent on FtsZ or PBP 2B. J Bacteriol, 2003. 185-2: p. 693-697.
Meima, R., C. Eschevins, S. Fillinger, A. Bolhuis, L.W. Hamoen, R. Dorenbos, W.J. Quax, J.M. van Dijl, R. Provvedi, I. Chen, D. Dubnau, and S. Bron, The bdbDC operon of Bacillus subtilis encodes thiol-disulfide oxidoreductases required for competence development. J Biol Chem, 2002. 277-9: p. 6994-7001.
Hamoen, L.W., W.K. Smits, A. de Jong, S. Holsappel, and O.P. Kuipers, Improving the predictive value of the competence transcription factor (ComK) binding site in Bacillus subtilis using a genomic approach. Nucleic Acids Res, 2002. 30-24: p. 5517-5528.
Risoen, P.A., O. Johnsborg, D.B. Diep, L. Hamoen, G. Venema, and I.F. Nes, Regulation of bacteriocin production in Lactobacillus plantarum depends on a conserved promoter arrangement with consensus binding sequence. Mol Genet Genomics, 2001. 265-1: p. 198-206.
Hamoen, L.W., B. Haijema, J.J. Bijlsma, G. Venema, and C.M. Lovett, The Bacillus subtilis competence transcription factor, ComK, overrides LexA-imposed transcriptional inhibition without physically displacing LexA. J Biol Chem, 2001. 276-46: p. 42901-42907.
Hamoen, L.W., A.F. Van Werkhoven, G. Venema, and D. Dubnau, The pleiotropic response regulator DegU functions as a priming protein in competence development in Bacillus subtilis. Proc Natl Acad Sci USA, 2000. 97-16: p. 9246-9251.
Kuipers, O.P., A. de Jong, S. Holsappel, S. Bron, J. Kok, and L.W. Hamoen, DNA-microarrays and food-biotechnology. Antonie Van Leeuwenhoek, 1999. 76-1-4: p. 353-355.
Duitman, E.H., L.W. Hamoen, M. Rembold, G. Venema, H. Seitz, W. Saenger, F. Bernhard, R. Reinhardt, M. Schmidt, C. Ullrich, T. Stein, F. Leenders, and J. Vater, The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase. Proc Natl Acad Sci USA, 1999. 96-23: p. 13294-13299.
Hamoen, L.W., A.F. Van Werkhoven, J.J. Bijlsma, D. Dubnau, and G. Venema, The competence transcription factor of Bacillus subtilis recognizes short A/T-rich sequences arranged in a unique, flexible pattern along the DNA helix. Genes Dev, 1998. 12-10: p. 1539-1550.
Turgay*, K., L.W. Hamoen*, G. Venema, and D. Dubnau, Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis. Genes Dev, 1997. 11-1: p. 119-128. *both authors equally contributed.
Elsner, A., H. Engert, W. Saenger, L. Hamoen, G. Venema, and F. Bernhard, Substrate specificity of hybrid modules from peptide synthetases. J Biol Chem, 1997. 272-8: p. 4814-4819.
Haijema, B.J., D. van Sinderen, K. Winterling, J. Kooistra, G. Venema, and L.W. Hamoen, Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis. Mol Microbiol, 1996. 22-1: p. 75-85.
van Sinderen, D., A. Luttinger, L. Kong, D. Dubnau, G. Venema, and L. Hamoen, comK encodes the competence transcription factor, the key regulatory protein for competence development in Bacillus subtilis. Mol Microbiol, 1995. 15-3: p. 455-462.
Hamoen, L.W., H. Eshuis, J. Jongbloed, G. Venema, and D. van Sinderen, A small gene, designated comS, located within the coding region of the fourth amino acid-activation domain of srfA, is required for competence development in Bacillus subtilis. Mol Microbiol, 1995. 15-1: p. 55-63.
Haijema, B.J., L.W. Hamoen, J. Kooistra, G. Venema, and D. van Sinderen, Expression of the ATP-dependent deoxyribonuclease of Bacillus subtilis is under competence-mediated control. Mol Microbiol, 1995. 15-2: p. 203-211.
van Sinderen, D., A. ten Berge, B.J. Hayema, L. Hamoen, and G. Venema, Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis. Mol Microbiol, 1994. 11-4: p. 695-703.
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Publications
2024
Gray, D. A., Wang, B., Sidarta, M., Cornejo, F. A., Wijnheijmer, J., Rani, R., Gamba, P., Turgay, K., Wenzel, M., Strahl, H., & Hamoen, L. W. (2024). Membrane depolarization kills dormant Bacillus subtilis cells by generating a lethal dose of ROS. Nature Communications, 15, Article 6877. https://doi.org/10.1038/s41467-024-51347-0 [details]- Kes, M. B. M. J., Wang, B., van Ulsen, P., Hamoen, L. W., & Luirink, J. (2024). Development of a split-luciferase assay to establish optimal protein secretion conditions for protein production by Bacillus subtilis. Microbiology - SGM, 170(6). https://doi.org/10.1099/mic.0.001460
- Wang, B., van der Kloet, F., Kes, M. B. M. J., Luirink, J., & Hamoen, L. W. (2024). Improving gene set enrichment analysis (GSEA) by using regulation directionality. Microbiology spectrum, e0345623. Advance online publication. https://doi.org/10.1128/spectrum.03456-23
2023
- Bohorquez, L. C., de Sousa, J., Garcia-Garcia, T., Dugar, G., Wang, B., Jonker, M. J., Noirot-Gros, M.-F., Lalk, M., & Hamoen, L. W. (2023). Metabolic and chromosomal changes in a Bacillus subtilis whiA mutant. Microbiology spectrum, 11(6), Article e0179523. https://doi.org/10.1128/spectrum.01795-23 [details]
- Wang, B., van der Kloet, F., & Hamoen, L. W. (2023). Induction of the CtsR regulon improves Xylanase production in Bacillus subtilis. Microbial Cell Factories, 22, Article 231. https://doi.org/10.1186/s12934-023-02239-3 [details]
2022
- Dugar, G., Hofmann, A., Heermann, D. W., & Hamoen, L. W. (2022). A chromosomal loop anchor mediates bacterial genome organization. Nature genetics, 54, 194–201. https://doi.org/10.1038/s41588-021-00988-8 [details]
- Ouyang, X., Hoeksma, J., Lubbers, R. J. M., Siersma, T. K., Hamoen, L. W., & den Hertog, J. (2022). Classification of antimicrobial mechanism of action using dynamic bacterial morphology imaging. Scientific Reports, 12, Article 11162. https://doi.org/10.1038/s41598-022-15405-1 [details]
- Saaki, T. N. V., Teng, Z., Wenzel, M., Ventroux, M., Carballido-Lόpez, R., Noirot-Gros, M. F., & Hamoen, L. W. (2022). SepF supports the recruitment of the DNA translocase SftA to the Z-ring. Molecular Microbiology, 117(5), 1263-1274. https://doi.org/10.1111/mmi.14906 [details]
- Wood, T. M., Zeronian, M. R., Buijs, N., Bertheussen, K., Abedian, H. K., Johnson, A. V., Pearce, N. M., Lutz, M., Kemmink, J., Siersma, T., Hamoen, L. W., Janssen, B. J. C., & Martin, N. I. (2022). Mechanistic insights into the C55-P targeting lipopeptide antibiotics revealed by structure-activity studies and high-resolution crystal structures. Chemical Science, 13(10), 2985-2991. https://doi.org/10.1039/d1sc07190d [details]
2021
- Michalik, S., Reder, A., Richts, B., Faßhauer, P., Mäder, U., Pedreira, T., Poehlein, A., van Heel, A. J., van Tilburg, A. Y., Altenbuchner, J., Klewing, A., Reuß, D. R., Daniel, R., Commichau, F. M., Kuipers, O. P., Hamoen, L. W., Völker, U., & Stülke, J. (2021). The Bacillus subtilis Minimal Genome Compendium. ACS Synthetic Biology, 10(10), 2767-2771. https://doi.org/10.1021/acssynbio.1c00339 [details]
- Syvertsson, S., Wang, B., Staal, J., Gao, Y., Kort, R., & Hamoen, L. W. (2021). Different Resource Allocation in a Bacillus subtilis Population Displaying Bimodal Motility. Journal of Bacteriology, 203(12), Article e00037-21. Advance online publication. https://doi.org/10.1128/JB.00037-21 [details]
- Wenzel, M., Celik Gulsoy, I. N., Gao, Y., Teng, Z., Willemse, J., Middelkamp, M., van Rosmalen, M. G. M., Larsen, P. W. B., van der Wel, N. N., Wuite, G. J. L., Roos, W. H., & Hamoen, L. W. (2021). Control of septum thickness by the curvature of SepF polymers. Proceedings of the National Academy of Sciences of the United States of America, 118(2), Article e2002635118. https://doi.org/10.1073/pnas.2002635118 [details]
- Wenzel, M., Dekker, M. P., Wang, B., Burggraaf, M. J., Bitter, W., van Weering, J. R. T., & Hamoen, L. W. (2021). A flat embedding method for transmission electron microscopy reveals an unknown mechanism of tetracycline. Communications biology, 4, Article 306. https://doi.org/10.1038/s42003-021-01809-8 [details]
2019
- Gray, D. A., Dugar, G., Gamba, P., Strahl, H., Jonker, M. J., & Hamoen, L. W. (2019). Extreme slow growth as alternative strategy to survive deep starvation in bacteria. Nature Communications, 10, Article 890. https://doi.org/10.1038/s41467-019-08719-8 [details]
2018
- Bohorquez, L. C., Surdova, K., Jonker, M. J., & Hamoen, L. W. (2018). The Conserved DNA Binding Protein WhiA Influences Chromosome Segregation in Bacillus subtilis. Journal of Bacteriology, 200(8), Article e00633-17. https://doi.org/10.1128/JB.00633-17 [details]
- Omardien, S., Drijfhout, J. W., Vaz, F. M., Wenzel, M., Hamoen, L. W., Zaat, S. A. J., & Brul, S. (2018). Bactericidal activity of amphipathic cationic antimicrobial peptides involves altering the membrane fluidity when interacting with the phospholipid bilayer. Biochimica et Biophysica Acta Biomembranes, 1860(11), 2404-2415. https://doi.org/10.1016/j.bbamem.2018.06.004 [details]
- Omardien, S., Drijfhout, J. W., van Veen, H., Schachtschabel, S., Riool, M., Hamoen, L. W., Brul, S., & Zaat, S. A. J. (2018). Synthetic antimicrobial peptides delocalize membrane bound proteins thereby inducing a cell envelope stress response. Biochimica et Biophysica Acta Biomembranes, 1860(11), 2416-2427. https://doi.org/10.1016/j.bbamem.2018.06.005 [details]
- Saaki, T. N. V., Strahl, H., & Hamoen, L. W. (2018). Membrane Curvature and the Tol-Pal Complex Determine Polar Localization of the Chemoreceptor Tar in Escherichia coli. Journal of Bacteriology, 200(9), Article e00658-17. https://doi.org/10.1128/JB.00658-17 [details]
- Saeloh, D., Tipmanee, V., Jim, K. K., Dekker, M. P., Bitter, W., Voravuthikunchai, S. P., Wenzel, M., & Hamoen, L. W. (2018). The novel antibiotic rhodomyrtone traps membrane proteins in vesicles with increased fluidity. PLoS Pathogens, 14(2), Article e1006876. https://doi.org/10.1371/journal.ppat.1006876 [details]
- Sauer, C., Ver Loren van Themaat, E., Boender, L. G. M., Groothuis, D., Cruz, R., Hamoen, L. W., Harwood, C. R., & van Rij, T. (2018). Exploring the Nonconserved Sequence Space of Synthetic Expression Modules in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1773-1784. https://doi.org/10.1021/acssynbio.8b00110 [details]
- Wenzel, M., Rautenbach, M., Vosloo, J. A., Siersma, T., Aisenbrey, C. H. M., Zaitseva, E., Laubscher, W. E., van Rensburg, W., Behrends, J. C., Bechinger, B., & Hamoen, L. W. (2018). The Multifaceted Antibacterial Mechanisms of the Pioneering Peptide Antibiotics Tyrocidine and Gramicidin S. MBio, 9(5), Article 00802-18. https://doi.org/10.1128/mBio.00802-18 [details]
- Wenzel, M., Vischer, N. O. E., Strahl, H., & Hamoen, L. W. (2018). Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes. BIO-PROTOCOL, 8(20), Article e3063. https://doi.org/10.21769/BioProtoc.3063 [details]
2017
- Gao, Y., Wenzel, M., Jonker, M. J., & Hamoen, L. W. (2017). Free SepF interferes with recruitment of late cell division proteins. Scientific Reports, 7, Article 16928. https://doi.org/10.1038/s41598-017-17155-x [details]
- Hamoen, L. W., & Wenzel, M. (2017). Editorial: Antimicrobial Peptides - Interaction with Membrane Lipids and Proteins. Frontiers in cell and developmental biology, 5, Article 4. https://doi.org/10.3389/fcell.2017.00004 [details]
- Saeloh, D., Wenzel, M., Rungrotmongkol, T., Hamoen, L. W., Tipmanee, V., & Voravuthikunchai, S. P. (2017). Effects of rhodomyrtone on Gram-positive bacterial tubulin homologue FtsZ. PeerJ, 5, Article e2962. https://doi.org/10.7717/peerj.2962 [details]
- de Jong, L., de Koning, E. A., Roseboom, W., Buncherd, H., Wanner, M. J., Dapic, I., Jansen, P. J., van Maarseveen, J. H., Corthals, G. L., Lewis, P. J., Hamoen, L. W., & de Koster, C. G. (2017). In-Culture Cross-Linking of Bacterial Cells Reveals Large-Scale Dynamic Protein-Protein Interactions at the Peptide Level. Journal of Proteome Research, 16(7), 2457-2471. https://doi.org/10.1021/acs.jproteome.7b00068 [details]
- den Blaauwen, T., Hamoen, L. W., & Levin, P. A. (2017). The divisome at 25: the road ahead. Current opinion in microbiology, 36, 85-94. Advance online publication. https://doi.org/10.1016/j.mib.2017.01.007 [details]
2016
- Derk te Winkel, J., Gray, D. A., Seistrup, K. H., Hamoen, L. W., & Strahl, H. (2016). Analysis of Antimicrobial-Triggered Membrane Depolarization Using Voltage Sensitive Dyes. Frontiers in cell and developmental biology, 4, Article 29. https://doi.org/10.3389/fcell.2016.00029 [details]
- Gamba, P., Hamoen, L. W., & Daniel, R. A. (2016). Cooperative Recruitment of FtsW to the Division Site of Bacillus subtilis. Frontiers in Microbiology, 7, Article 1808. https://doi.org/10.3389/fmicb.2016.01808 [details]
- Kloosterman, T. G., Lenarcic, R., Willis, C. R., Roberts, D. M., Hamoen, L. W., Errington, J., & Wu, L. J. (2016). Complex polar machinery required for proper chromosome segregation in vegetative and sporulating cells of Bacillus subtilis. Molecular Microbiology, 101(2), 333-350. Advance online publication. https://doi.org/10.1111/mmi.13393 [details]
- Müller, A., Wenzel, M., Strahl, H., Grein, F., Saaki, T. N. V., Kohl, B., Siersma, T., Bandow, J. E., Sahl, H-G., Schneider, T., & Hamoen, L. W. (2016). Daptomycin inhibits cell envelope synthesis by interfering with fluid membrane microdomains. Proceedings of the National Academy of Sciences of the United States of America, 113(45), E7077–E7086. Advance online publication. https://doi.org/10.1073/pnas.1611173113 [details]
- Sauer, C., Syvertsson, S., Bohorquez, L. C., Cruz, R., Harwood, C. R., van Rij, T., & Hamoen, L. W. (2016). Effect of Genome Position on Heterologous Gene Expression in Bacillus subtilis: An Unbiased Analysis. ACS Synthetic Biology, 5(9), 942-947. Advance online publication. https://doi.org/10.1021/acssynbio.6b00065 [details]
- Syvertsson, S., Vischer, N. O. E., Gao, Y., & Hamoen, L. W. (2016). When Phase Contrast Fails: ChainTracer and NucTracer, Two ImageJ Methods for Semi-Automated Single Cell Analysis Using Membrane or DNA Staining. PLoS ONE, 11(3), Article e0151267. https://doi.org/10.1371/journal.pone.0151267 [details]
2015
- Gamba, P., Jonker, M. J., & Hamoen, L. W. (2015). A Novel Feedback Loop That Controls Bimodal Expression of Genetic Competence. PLOS Genetics, 11(6), Article e1005047. Advance online publication. https://doi.org/10.1371/journal.pgen.1005047 [details]
- Gamba, P., Rietkötter, E., Daniel, R. A., & Hamoen, L. W. (2015). Tetracycline hypersensitivity of an ezrA mutant links GalE and TseB (YpmB) to cell division. Frontiers in Microbiology, 6, Article 346. https://doi.org/10.3389/fmicb.2015.00346 [details]
- Strahl, H., Ronneau, S., Solana González, B., Klutsch, D., Schaffner-Barbero, C., & Hamoen, L. W. (2015). Transmembrane protein sorting driven by membrane curvature. Nature Communications, 6, Article 8728. https://doi.org/10.1038/ncomms9728 [details]
- Strahl, H., Turlan, C., Khalid, S., Bond, P. J., Kebalo, J. M., Peyron, P., Poljak, L., Bouvier, M., Hamoen, L., Luisi, B. F., & Carpousis, A. J. (2015). Membrane recognition and dynamics of the RNA degradosome. PLOS Genetics, 11(2), Article e1004961. Advance online publication. https://doi.org/10.1371/journal.pgen.1004961 [details]
2014
- Halbedel, S., Kawai, M., Breitling, R., & Hamoen, L. W. (2014). SecA is required for membrane targeting of the cell division protein DivIVA in vivo. Frontiers in Microbiology, 5, 58. https://doi.org/10.3389/fmicb.2014.00058 [details]
- Strahl, H., Burmann, F., & Hamoen, L. W. (2014). The actin homologue MreB organizes the bacterial cell membrane. Nature Communications, 5, 3442. https://doi.org/10.1038/ncomms4442 [details]
2013
- Duman, R., Ishikawa, S., Celik, I., Strahl, H., Ogasawara, N., Troc, P., Löwe, J., & Hamoen, L. W. (2013). Structural and genetic analyses reveal the protein SepF as a new membrane anchor for the Z ring. Proceedings of the National Academy of Sciences of the United States of America, 110(48), E4601-E4610. https://doi.org/10.1073/pnas.1313978110 [details]
- Surdova, K., Gamba, P., Claessen, D., Siersma, T., Jonker, M. J., Errington, J., & Hamoen, L. W. (2013). The conserved DNA-binding protein WhiA is involved in cell division in Bacillus subtilis. Journal of Bacteriology, 195(24), 5450-5460. Advance online publication. https://doi.org/10.1128/JB.00507-13 [details]
- van Baarle, S., Celik, I. N., Kaval, K. G., Bramkamp, M., Hamoen, L. W., & Halbedel, S. (2013). Protein-protein interaction domains of Bacillus subtilis DivIVA. Journal of Bacteriology, 195(5), 1012-1021. Advance online publication. https://doi.org/10.1128/JB.02171-12 [details]
2012
- Strahl, H., & Hamoen, L. W. (2012). Finding the corners in a cell. Current opinion in microbiology, 15(6), 731-736. https://doi.org/10.1016/j.mib.2012.10.006 [details]
- Wenzel, M., Kohl, B., Münch, D., Raatschen, N., Albada, H. B., Hamoen, L., Metzler-Nolte, N., Sahl, H. G., & Bandow, J. E. (2012). Proteomic response of Bacillus subtilis to lantibiotics reflects differences in interaction with the cytoplasmic membrane. Antimicrobial Agents and Chemotherapy, 56(11), 5749-5757. https://doi.org/10.1128/AAC.01380-12 [details]
2018
- Corthals, G., de Jong, L., de Koning, E. A., Roseboom, W., Buncherd, H., Wanner, M., Dapic, I., Jansen, P., van Maarseveen, J., Lewis, P., Hamoen, L., & de Koster, C. (2018). Identification of dynamic protein interactions from live cells. FEBS OPEN BIO, 8(S1), 17-18. Article S.32-5. https://doi.org/10.1002/2211-5463.12449 [details]
2024
- Han, Y. (2024). Ribosome profiling reveals ribosome pausing in Bacillus subtilis. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Liu, X. (2024). Escherichia coli cell envelope growth by spatiotemporal coordination of peptidoglycan synthesis and degradation. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Pineda Hernández, H. (2024). On the quest to study polyploidy in cyanobacteria. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Teng, Z. (2024). Characterization of unknown Bacillus subtilis genes with transposon sequencing. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
2022
- Consoli, E. (2022). Spatiotemporal topography of the BAM complex in relation to peptidoglycan and cell division. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Wang, B. (2022). Secretion stress, ROS stress and gene fitness in Bacillus subtilis. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Wang, J. (2022). The cytoskeletal proteins MreB and FtsZ and the peptidoglycan hydrolase PBP4 act together in longitudinally dividing bacterium Candidatus Thiosymbion oneisti. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
2021
- Caicedo Burbano, S. P. (2021). Improving the synthetic biology toolbox of Synechocystis sp. PCC 6803. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Wu, W. (2021). Photoautotrophic overproduction of mannitol in Synechocystis sp. PCC6803 using osmotic pressure as a driving force. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Zdouc, M. M. (2021). Metabolomic and genomic investigation into the actinomycete genus Planomonospora. [Thesis, externally prepared, Universiteit van Amsterdam]. [details]
2020
2019
- Gao, Y. (2019). Cell division in walled Bacillus subtilis and cell wall-lacking Acholeplasma laidlawii. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Liu, X. (2019). Spatio-temporal organization of the lipid II flippase MurJ and the lateral wall synthesizing proteins RodA and PBP2. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Meiresonne, N. Y. (2019). Illuminating protein interactions in the periplasm. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Saaki, T. N. V. (2019). The minimalistic divisome reveals power of the cell division machinery. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
2024
- Wang, B., Hamoen, L. & van der Kloet, F. (2024). Additional file 2 of Induction of the CtsR regulon improves Xylanase production in Bacillus subtilis. Figshare. https://doi.org/10.6084/m9.figshare.24540866.v1
2023
- Wang, B., van der Kloet, F. & Hamoen, L. W. (2023). Additional file 1 of Induction of the CtsR regulon improves Xylanase production in Bacillus subtilis. Figshare. https://doi.org/10.6084/m9.figshare.24540607.v1
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Gray, D. A., Wang, B., Sidarta, M., Cornejo, F. A., Wijnheijmer, J., Rani, R., Gamba, P., Turgay, K., Wenzel, M., Strahl, H., & Hamoen, L. W. (2024). Membrane depolarization kills dormant Bacillus subtilis cells by generating a lethal dose of ROS. Nature Communications, 15, Article 6877.